uts at the airlines Eurowings and Lufthansa CityLine. In a statement, the union said it had called on its members to strike the subsidiaries of Deutsche Lufthansa AG from noon (1000 GMT) Tuesday through midnight Wednesday.
The union has been waging a series of strikes at the airlines amid an ongoing dispute over pay increases for pilots. The last walkout led to more than 600 flights in Germany being canceled.
The union said it called the strikes because Lufthansa, Germany's biggest airline, had not delivered what it called a negotiable offer in the talks, which have been ongoing for months.
The union said 99 percent of its members voted in favor of the walkouts. The strikes will airports including Frankfurt - continental Europe's second largest after Paris Charles de Gaulle - along with Munich, Stuttgart, Hamburg, Berlin, Nuremberg, Duesseldorf and Hannover.
Lufthansa spokesman Michael Lamberti said the strike was "not comprehensible," and that the airline had presented a good offer.
"We're trying to be as accommodating as possible," he said of the talks.
Lamberti said it was immediately unclear how many flights could be affected, as well as connections with other airlines.
More than 1,000 CityLine and Eurowings pilots were set to walk off their jobs.
Lamberti said domestic Lufthansa tickets could be used on Deutsche Bahn, the German rail carrier.
Lufthansa has also been plagued by strikes of service union ver.di which represents ground handlers and cabin staff.
On July 1, about 4,500 ver.di union members walked off their jobs, disrupting flights to and from airports across Germany in an effort to win more pay, union organizers said.
That strike mainly involved ground personnel from Frankfurt Airport, but employees in Munich, Stuttgart and Cologne also walked off their jobs for up to four hours.
The Ver.di union was seeking a 9.8 percent pay hike for Lufthansa's roughly 60,000 ground and cabin personnel, while the airline has offered a total of 5.5 percent in graded increases over the year.
Shares of Lufthansa were down 4.6 percent at £á14.53 (US$22.96).